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Hoefer hoefer slab gel dryer
Hoefer Slab Gel Dryer, supplied by Hoefer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/slab+gel+dryer/dryer+gel/pm41786720-268-24-24
Average 86 stars, based on 1 article reviews
hoefer slab gel dryer - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Ubiquitin Proteomics:

Article Title: RING finger proteins of infectious spleen and kidney necrosis virus (ISKNV) function as ubiquitin ligase enzymes.
Article Snippet: Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of nlarged and damaged spleen and kidney.. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading rames (ORFs) of the ISKNV genome, ORF12, ORF65, ORF66, ORF99 and ORF111, which encode RING finger proteins (RFPs).. We assessed he ubiquitin ligase (E3) activity of these recombinant RFPs fused to maltose-binding protein (MBP) using an in vitro ubiquitination assay and emonstrated that ORF12, ORF65, ORF66 and ORF111 possess the E3 activity in the presence of ubiquitin activating enzyme (E1), ubiquitin onjugating enzyme (E2), ubiquitin and zinc ion.

Electrophoresis:

Article Title: RING finger proteins of infectious spleen and kidney necrosis virus (ISKNV) function as ubiquitin ligase enzymes.
Article Snippet: Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of nlarged and damaged spleen and kidney.. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading rames (ORFs) of the ISKNV genome, ORF12, ORF65, ORF66, ORF99 and ORF111, which encode RING finger proteins (RFPs).. We assessed he ubiquitin ligase (E3) activity of these recombinant RFPs fused to maltose-binding protein (MBP) using an in vitro ubiquitination assay and emonstrated that ORF12, ORF65, ORF66 and ORF111 possess the E3 activity in the presence of ubiquitin activating enzyme (E1), ubiquitin onjugating enzyme (E2), ubiquitin and zinc ion.

SDS-Gel:

Article Title: RING finger proteins of infectious spleen and kidney necrosis virus (ISKNV) function as ubiquitin ligase enzymes.
Article Snippet: Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of nlarged and damaged spleen and kidney.. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading rames (ORFs) of the ISKNV genome, ORF12, ORF65, ORF66, ORF99 and ORF111, which encode RING finger proteins (RFPs).. We assessed he ubiquitin ligase (E3) activity of these recombinant RFPs fused to maltose-binding protein (MBP) using an in vitro ubiquitination assay and emonstrated that ORF12, ORF65, ORF66 and ORF111 possess the E3 activity in the presence of ubiquitin activating enzyme (E1), ubiquitin onjugating enzyme (E2), ubiquitin and zinc ion.

Autoradiography:

Article Title: RING finger proteins of infectious spleen and kidney necrosis virus (ISKNV) function as ubiquitin ligase enzymes.
Article Snippet: Infectious spleen and kidney necrosis virus (ISKNV) is the etiological agent that causes a pandemic and severe disease in fish characteristic of nlarged and damaged spleen and kidney.. To identify viral proteins involved in infection and pathogenesis, we characterized five open reading rames (ORFs) of the ISKNV genome, ORF12, ORF65, ORF66, ORF99 and ORF111, which encode RING finger proteins (RFPs).. We assessed he ubiquitin ligase (E3) activity of these recombinant RFPs fused to maltose-binding protein (MBP) using an in vitro ubiquitination assay and emonstrated that ORF12, ORF65, ORF66 and ORF111 possess the E3 activity in the presence of ubiquitin activating enzyme (E1), ubiquitin onjugating enzyme (E2), ubiquitin and zinc ion.

Two-Dimensional Gel Electrophoresis:

Article Title: Cancer associated fibroblasts transfer lipids and proteins to cancer cells through cargo vesicles supporting tumor growth.
Article Snippet: .. Gels were dried on a Slab Gel Dryer (Hoefer, model CD 2000) and autoradiographed for the appropriate time (2 days for fibroblasts 2DE gel and 15 days for DU145 2DE gel). .. Images were acquired on a Cyclone Storage Phosphor Scanner (Perkin Elmer). (A) Primary fibroblasts of prostatic origin (HPFs) and fibroblasts activated with DU145 or unlabeled healthy fibroblasts in a 2:1 ratio in starvationmedium. (B) Primary fibroblasts of abeled with CFDA-SE and then plated in coculture with unlabeled healthy fibroblasts in a y.

Staining:

Article Title: Protein Kinase C-Mediated Phosphorylation of a Single Serine Residue on the Rat Glial Glutamine Transporter SN1 Governs Its Membrane Trafficking
Article Snippet: .. Gels were stained in Coomassie blue (Sigma), destained, and dried in a Hoefer Slab Gel Dryer. .. Incorporated radioactivity was analyzed in a Typhoon 9210 Imager using ImageQuant 5.1 software (Molecular Dynamics).

other:

Article Title: Sequencing of the Chlamydophila psittaci ompA Gene Reveals a New Genotype, E/B, and the Need for a Rapid Discriminatory Genotyping Method
Article Snippet: Afterwards, the gel was dried in a slab gel dryer (Hoefer; Amersham Biosciences) and analyzed 24 h later using the Typhoon 9200 phosphoimager (Amersham Biosciences).



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